Disposable Immuno-Sensor For Detection Of Antibiotic Residues In Livestock Produces
DISPOSABLE IMMUNO-SENSOR FOR DETECTION OF ANTIBIOTIC RESIDUES IN LIVESTOCK PRODUCES
Introduction
Most of the antibiotic products were originally introduced to improve human and animal health. Abuse of these antibiotic can have harmful effects on animal and humans, where it can accumulate in animal tissues and can enter the food chain once the animal is slaughtered. Various regulatory bodies have acted to control the use of these antibiotics in farming and subsequently creating the need for sensitive, reliable, cost-effective methods for the detection of antibiotics. Simple disposable electrochemical immuno-biosensor using screen-printed electrode (SPE) coated with antibodies was developed for antibiotic detection. This innovation was found to be more efficient, sensitive, specific and simple to use. It is capable of handling many samples at lower costs. The innovation is also very useful in development of commercial biosensor for quick detection of chemical residues in poultry and other livestock meat.
Description of Innovation
This innovation is based on the specific antibody against streptomycin antibiotic that was coated on the surface of the screen printed electrode which was connected to the electrochemical analyser (potentiostat), together with a Ag/AgCl reference electrode and a Pt auxiliary electrode. The three electrodes were placed in a stirred 5 ml vessel. The electrodes were operated using the cronoamperometric method, which has time base modes in seconds.
This innovation is based on the specific antibody against streptomycin antibiotic that was coated on the surface of the screen printed electrode which was connected to the electrochemical analyser (potentiostat), together with a Ag/AgCl reference electrode and a Pt auxiliary electrode. The three electrodes were placed in a stirred 5 ml vessel. The electrodes were operated using the cronoamperometric method, which has time base modes in seconds.
a) Antibodies are in a more stable environment upon immobilization in the polymeric framework, thereby creating a stage and thus protecting the antibody from aggregation and unfolding on the surface of the SPE.
b) Antibody could recognize specifically their antigens. Thus in this case coated SPE could detect their specific antibiotics.
Source : http://www.mardi.my
Categories:
antibiotic,
disposable,
new invention
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